KMID : 0928520070170010011
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Korean Journal of Lipidology 2007 Volume.17 No. 1 p.11 ~ p.18
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PPAR¥ãActivation Abolishes LDL-induced Proliferation of Human Aortic Smooth Muscle Cells via Erk1/2-mediated Down-regulation of Egr-1
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Heo Kyung-Sun
Kim Dong-Uk Nam Mi-Young Baek Seung-Tae Lee Hye-Mi Hwang Hye-Rim Park Song-Kyu Park Young-Woo Lee Sung-Hou Myung Chang-Seon Hoe Kwang-Lae
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Abstract
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Objective: Native LDL would be a mitogenic and chemotactic stimulus of VSMC proliferation in the atherosclerotic lesion where endothelial disruption occurred. Among peroxisome proliferative-activated receptors (PPARs) ¥á, ¥ã, and ¥â/¥ä, as transcription factors exerting modulatory action in vascular cells, PPAR¥ã exhibits antiatherogenic properties and its agonists are applied to patients in cardiovascular disease. The aim of the study was to investigate the effects of PPAR¥ã agonist troglitazone (TG) on LDL-induced cell proliferation in human aortic smooth muscle cells (hAoSMCs).
Methods: Human AoSMCs were treated with LDL in dose- and time-dependent manners. Cell proliferation was determined by WST-1 and BrdU incorporation assays. The activity of extracellular signal-regulated kinase1/2 (Erk1/2) and early growth response factor 1 (Egr-1) was measured by Western blot using phospho-Erk and Egr-1 antibodies. Specific inhibition of TG on Egr-1 transcriptional expression was determined by Northern blot analysis.
Results: Treatment of hAoSMCs with LDL increased cell proliferation in dose- and time-dependent manners. LDL-induced cell proliferation was accompanied by Erk1/2 activation and sequential up-regulation of Egr-1. PPAR¥ã activation by TG exerted its inhibitory effects on LDL-induced cell proliferation via Erk1/2-dependent down-regulation of Egr-1 in hAoSMCs.
Conclusion: PPAR¥ã activation exerts its beneficial effects on LDL-induced cell proliferation of hAoSMCs.
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KEYWORD
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Low-density lipoproteins, Egr-1, MAP kinase, PPAR, Smooth muscle cell, Troglitazone
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